RSBI would like to thank all of the speakers and trainers for joining us yet again for another NGS training in Romania, and Net-Connect Group for kindly and generously sponsoring the event.

The average rating from 28 participants was 4.3/5. All of the talks were well received by the public, but they were most impressed with Viorica Chelban of University College London, who talked about her work on gene discovery and clinical validation of NGS results in neurological disorders.

Several trainees wrote in their feedback forms that the training should have run for 5 days instead of 3. Other comments: “You are amazing! Keep up the great work.” ” try to organize many other workshops like this one.” “Keep it up, guys! Those three days I felt like having access to the education I’ve always wanted – cutting edge research, novel technologies, practical sessions, challenging subjects and open minded mentors”

Next-Generation Sequencing Data Analysis

Beer and science event


25-27 February 2019


Platform for Research on Systemic Biology and Ecology, Faculty of Biology, Bogdan Petriceicu Hasdeu Street, Bucharest.
Conference Room, Ground Floor.

In Romanian: “Platforma de Cercetare in Biologie si Ecologie Sistemica, Facultatea de Biologie, Strada Bogdan Petriceicu Hasdeu, București; Sala de conferinte, Parter”


Marieta Costache (University of Bucharest, Romania)
Dan Mihăilescu (University of Bucharest, Romania)
Sorina Dinescu (University of Bucharest, Romania)
Robi Tăcutu (Institutul de Biochimie al Academiei Române, Bucharest, Romania)
Valeriu Ohan (Cambridge, UK)
Alexandru Salceanu (Bucharest, Romania)
Monica Abrudan (Cambridge, UK)

Research Institute of the University of Bucharest


REGISTRATION IS NOW CLOSED. If you sent and application and you still haven’t heard from us, please drop us an email.

Applicants will be asked to write a motivational letter, in which they explain 1. why the course is important for their career development 2. why they should be the ones selected to participate in the training, and not others.
In the past, we have received three times more applications than the number of available spots in the trainings. To avoid disappointment, please make sure that you do your best to convince us that you are the best person to participate in the training.

A registration fee of 50 euros is required from academia participants to cover meals and dinner. Bursaries are available (up to 10 places; with 50% reduction or full fee exemption), and are awarded on merit and motivation. Mention in the registration form if you intend to apply for it.


A fundamental understanding of Molecular Biology is assumed.
In order to benefit most from the training, the participants are encouraged to refresh their knowledge of Statistics, Unix command line, R and/or Python.
Suggested resources:

Speakers and trainers

Viorica Chelban (University College London, Institute of Neurology, London, UK)
Hugues Richard (Pierre and Marie Curie University, Paris, France)
Petr Daněček (University of Cambridge, Cambridge, UK)
Valeriu Ohan (Wellcome Sanger Institute, Cambridge, UK)
Aleksandra Świercz (University of Technology, Poznan, Poland)
Bogdan Mirăuță (European Bioinformatics Institute, Cambridge, UK)
Diana Miclea (Cluj-Napoca Regional Genetic Centre, Cluj-Napoca, Romania)
Robi Tăcutu (Institutul de Biochimie al Academiei Române, Bucharest, Romania)
Yuliya Sytnikova (Harvard Medical School, USA)



Day 1: DNA sequencing

9:00 – 9:15Welcome noteRobi Tăcutu
9:15 – 10:30Hands-on: Introduction to Unix environmentValeriu Ohan
10:30 – 11:00Coffee Break
11:00 – 11:30Theory: File formats, QC
Petr Daněček
11:30 – 12:00Hands-on: FASTA, FASTQ filesValeriu Ohan
12:00 – 13:00Lunch
13:00 – 14:00Theory: AlignmentPetr Daněček
14:00 – 14:30Hands-on: SAM, BAM filesValeriu Ohan
14:30 – 15:00Coffee Break
15:00 – 16:00Theory: Variant callingPetr Daněček
16:00 – 16:30Hands-on: VCF, BCF filesValeriu Ohan
16:30 – 17:30Hands-on: An NGS pipeline exerciseValeriu Ohan, Petr Daněček
18:30Beer and Science event. Location: The Pub, in Universitatii Square.

Day 2: RNA sequencing

9:00 – 9:50Talk: NGS in rare genetic disease discoveryViorica Chelban
9:50 – 10:10Talk: PTC test resultsPetr Daněček
10:10 – 10:30Theory: An introduction to RNA-seqHugues Richard
10:30 – 11:00Coffee Break
11:00 – 12:00Hands-on: RNA-seq (I)Hugues Richard
12:00 – 13:00Lunch
13:00 – 14:00Hands-on: RNA-seq (II)Hugues Richard
14:00 – 14:30Theory: Analysis of RNA-seq data for a research paper. Essential steps from read counts to results.Bogdan Mirăuță
14:30 – 15:00Coffee break
15:00 – 17:00Hands-on: Analysis of RNA-seq data for a research paper. Essential steps from read counts to results.
Bogdan Mirăuță
19:00Festive dinner: Caru cu Bere, Strada Stavropoleos 5, Bucharest.

Day 3: Genome assemblies

9:00 – 10:30Theory: Genome AssemblyAleksandra Świercz
10:30 – 11:00Coffee Break
11:00 – 12:00Hands-on: Genome AssemblyAleksandra Świercz
12:00 – 13:00Lunch
13:00 – 13:30Talk: Genomic technologies for the diagnosis of rare endocrine disorders in Cluj-Napoca Regional Genetic CentreDiana Miclea
13:30 – 14:00Talk: Sequencing with long read technologies (Oxford Nanopore and Pacific Biosciences)Aleksandra Świercz
14:00 – 14:30Talk: Uncovering chromatin signatures with NGS approachesYuliya Sytnikova
14:30 – 15:00Coffee break
15:00 – 15:15 Talk: Gerontomics, surfing omics data for ageing researchRobi Tăcutu
15:15 – 15:30 Talk: Gerontomics – an NGS application: analyzing the de novo transcriptome of the long-lived gray whale and comparison with other speciesDmitry Toren

Beer and science event

‘Beer and science’ is an informal event, where scientists present a science topic in 5 minutes, in a pub, to their peers, but also to non-scientists. Our goal is to promote interdisciplinary talks between speacialists in Biology, Medicine, Computer Science and Engineering.

We encourage simple and easy to digest presentations.

At the end of each presentation, we encourage participants to join in the discussions and ask questions. This is a good opportunity for scientists to discuss their faced issues with the broader community. Discussions between the speaker and public can generate ideas for solving existing problems. Also, it can lead to new research ideas.

The event will take place at The Pub, in Universitatii Square.

Please sign up for the event using this form:

Categories: RSBI EventsFuture Events